UV-activated conversion of Hoechst 33258, DAPI, and Vybrant DyeCycle fluorescent dyes into blue-excited, green-emitting protonated forms
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چکیده
منابع مشابه
Binding Mechanisms of Methylene Blue and Hoechst 33258 with Dna
In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It wa...
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Single Molecule Localization Microscopy (SMLM) is a recently emerged optical imaging method that was shown to achieve a resolution in the order of tens of nanometers in intact cells. Novel high resolution imaging methods might be crucial for understanding of how the chromatin, a complex of DNA and proteins, is arranged in the eukaryotic cell nucleus. Such an approach utilizing switching of a fl...
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An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
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Several approaches have been described to fluorescently label and image DNA and chromatin in situ on the single-molecule level. These superresolution microscopy techniques are based on detecting optically isolated, fluorescently tagged anti-histone antibodies, fluorescently labeled DNA precursor analogs, or fluorescent dyes bound to DNA. Presently they suffer from various drawbacks such as low ...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2013
ISSN: 1552-4922
DOI: 10.1002/cyto.a.22260